| Immunoassay Kit Format |
Uncoated ELISA Kit |
| Content And Storage |
Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples 10 x Coating Buffer: Buffer for plating the Capture Antibody 5 x ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme Enzyme: Pre-titrated Avidin-HRP Substrate: 1 x TMB Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards 96-well Plate: Corning Costar 9018 (included with product Cat. No. ending in suffixes -22, -76, -86) |
| Target Species |
Mouse |
| Conjugate |
HRP |
| Gene Symbol |
Il5 |
| Total Assay Time |
24 hr 30 min |
| Sample Volume |
Lavage Fluid 100 μL, Serum 100 μL, Supernatant 100 μL |
| Assay Sensitivity |
8 pg/mL |
| Storage Requirements |
2-8°C |
| Assay Range |
8-500 pg/mL |
| Test Time |
1 hr 45 min |
| Accession Number |
P04401 |
| Regulatory Status |
RUO |
| Target |
IL-5 |
| Kit Contents |
Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| Gene Alias |
B-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRFB-cell differentiation factor I; B-cell growth factor II; BCGF-II; colony-stimulating factor, eosinophil; cytotoxic T-lymphocyte inducer; EDF; Eosinophil differentiation factor; IL5; IL-5; ILN; Interleukin; Interleukin 5; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); Interleukin5; interleukin-5; T-cell replacing factor; TRF; CANDF6; IL-17F; ML-1; ML1 |
| For Use With (Equipment) |
Absorbance Microplate Reader |
| Product Type |
ELISA |
| Gene ID (Entrez) |
16191 |
| Sample Type |
Lavage Fluid,Serum,Supernatant |
| Label or Dye |
Biotin |